GLMN monoclonal antibody (M01), clone 1C12
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More Files
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Western Blot (Cell lysate)
GLMN monoclonal antibody (M01), clone 1C12. Western Blot analysis of GLMN expression in HL-60 ( Cat # L014V1 ).
Western Blot (Cell lysate)
GLMN monoclonal antibody (M01), clone 1C12. Western Blot analysis of GLMN expression in Jurkat ( Cat # L017V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of GLMN expression in transfected 293T cell line by GLMN monoclonal antibody (M01), clone 1C12.
Lane 1: GLMN transfected lysate(68.2 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of GLMN transfected lysate using anti-GLMN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLMN MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GLMN is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to GLMN on HeLa cell. [antibody concentration 20 ug/ml]
Western Blot detection against Immunogen (91.08 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GLMN.
Immunogen
GLMN (AAH01257, 1 a.a. ~ 594 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAVEELQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTDQLLEIIQNEKNKVIIKNMGWNLVGPVVRCLLCKDKEDSKRKVYFLIFDLLVKLCNPKELLLGLLELIEEPSGKQISQSILLLLQPLQTVIQKLHNKAYSIGLALSTLWNQLSLLPVPYSKEQIQMDDYGLCQCCKALIEFTKPFVEEVIDNKENSLENEKLKDELLKFCFKSLKCPLLTAQFFEQSEEGGNDPFRYFASEIIGFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENKQLADSMASLAYLVFVQGIHIDQLPMVLSPLYLLQFNMGHIEVFLQRTEESVISKGLELLENSLLRIEDNSLLYQYLEIKSFLTVPQGLVKVMTLCPIETLRKKSLAMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFIIQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFLPEGAETDLLQNSDRIMASLNLLRYLVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIKNSQEAQKSKDLCSITVSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEELIEIKTKSTSEENIGIK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (83)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (91.08 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GLMN monoclonal antibody (M01), clone 1C12. Western Blot analysis of GLMN expression in HL-60 ( Cat # L014V1 ).Western Blot (Cell lysate)
GLMN monoclonal antibody (M01), clone 1C12. Western Blot analysis of GLMN expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of GLMN expression in transfected 293T cell line by GLMN monoclonal antibody (M01), clone 1C12.
Lane 1: GLMN transfected lysate(68.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of GLMN transfected lysate using anti-GLMN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLMN MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GLMN is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GLMN on HeLa cell. [antibody concentration 20 ug/ml] -
Gene Info — GLMN
Entrez GeneID
11146GeneBank Accession#
BC001257Protein Accession#
AAH01257Gene Name
GLMN
Gene Alias
FAP, FAP48, FAP68, FKBPAP, GLML, GVM, VMGLOM
Gene Description
glomulin, FKBP associated protein
Gene Ontology
HyperlinkGene Summary
This gene encodes a phosphorylated protein that is a member of a Skp1-Cullin-F-box-like complex. The protein is essential for normal development of the vasculature and mutations in this gene have been associated with glomuvenous malformations, also called glomangiomas. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq
Other Designations
FK506-binding protein-associated protein|OTTHUMP00000011939|glomulin|venous malformation with glomus cells
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