ZWINT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ZWINT transfected lysate.
Western Blot
Lane 1: ZWINT transfected lysate ( 31.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ZWINT full-length
Host
Human
Theoretical MW (kDa)
31.2
Interspecies Antigen Sequence
Mouse (59); Rat (58)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ZWINT antibody (H00011130-B01) by Western Blots.
SDS-PAGE Gel
ZWINT transfected lysate.
Western Blot
Lane 1: ZWINT transfected lysate ( 31.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ZWINT
Entrez GeneID
11130GeneBank Accession#
BC020979.1Protein Accession#
-Gene Name
ZWINT
Gene Alias
HZwint-1, KNTC2AP, MGC117174, ZWINT1
Gene Description
ZW10 interactor
Omim ID
609177Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019626|OTTHUMP00000019628|human ZW10 interacting protein-1
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Interactome
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Disease
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