CD160 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CD160 full-length
Host
Human
Theoretical MW (kDa)
19.8
Interspecies Antigen Sequence
Mouse (66)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CD160 antibody (H00011126-D01P) by Western Blots.
SDS-PAGE Gel
CD160 transfected lysate.
Western Blot
Lane 1: CD160 transfected lysate ( 19.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CD160
Entrez GeneID
11126GeneBank Accession#
NM_007053Protein Accession#
AAH14465.1Gene Name
CD160
Gene Alias
BY55, FLJ46513, NK1, NK28
Gene Description
CD160 molecule
Omim ID
604463Gene Ontology
HyperlinkGene Summary
CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq
Other Designations
CD160 antigen|OTTHUMP00000015585|natural killer cell receptor, immunoglobulin superfamily member
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