MTHFD2 monoclonal antibody (M01), clone 4G7-2G3
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More Files
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Western Blot (Cell lysate)
MTHFD2 monoclonal antibody (M01), clone 4G7-2G3 Western Blot analysis of MTHFD2 expression in HepG2 ( Cat # L019V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody (M01), clone 4G7-2G3.
Lane 1: MTHFD2 transfected lysate(26.8 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MTHFD2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MTHFD2 is 1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (61.93 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MTHFD2.
Immunogen
MTHFD2 (AAH01548, 16 a.a. ~ 344 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN
Host
Mouse
Reactivity
Human
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (61.93 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MTHFD2 monoclonal antibody (M01), clone 4G7-2G3 Western Blot analysis of MTHFD2 expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody (M01), clone 4G7-2G3.
Lane 1: MTHFD2 transfected lysate(26.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MTHFD2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MTHFD2 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — MTHFD2
Entrez GeneID
10797GeneBank Accession#
BC001548Protein Accession#
AAH01548Gene Name
MTHFD2
Gene Alias
NMDMC
Gene Description
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Omim ID
604887Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq
Other Designations
NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase|methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase|methylene tetrahydrofolate dehydrogenase 2
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Interactome
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Pathway
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Disease
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Publication Reference
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One carbon metabolism in human lung cancer.
Sha Yao, Luogen Peng, Omar Elakad, Stefan Küffer, Marc Hinterthaner, Bernhard C Danner, Alexander von Hammerstein-Equord, Philipp Ströbel, Hanibal Bohnenberger.
Translational Lung Cancer Research 2021 Jun; 10(6):2523.
Application：IHC, Human, Human lung cancer tissues.
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Identification of theranostic factors for patients developing metastasis after surgery for early-stage lung adenocarcinoma.
Wei-Chung Cheng, Chun-Yu Chang, Chia-Chien Lo, Chih-Ying Hsieh, Ting-Ting Kuo, Guan-Chin Tseng, Sze-Ching Wong, Shu-Fen Chiang, Kevin Chih-Yang Huang, Liang-Chuan Lai, Tzu-Pin Lu, K S Clifford Chao, Yuh-Pyng Sher.
Theranostics 2021 Jan; 11(8):3661.
Application：WB-Tr, Human, A-549, CL1-0 cells.
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One carbon metabolism in human lung cancer.
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