MGEA5 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of MGEA5 transfected lysate using rabbit polyclonal anti-MGEA5 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse polyclonal anti-MGEA5.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of MGEA5 transfected lysate using rabbit polyclonal anti-MGEA5 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse polyclonal anti-MGEA5.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-MGEA5 (300 ul)
2. Antibody pair for WB: mouse polyclonal anti-MGEA5 (50 ul)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — MGEA5
Entrez GeneID
10724Gene Name
MGEA5
Gene Alias
FLJ11229, FLJ23355, KIAA0679, MEA5, NCOAT, OGA
Gene Description
meningioma expressed antigen 5 (hyaluronidase)
Omim ID
604039Gene Ontology
HyperlinkGene Summary
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM
Other Designations
O-GlcNAcase|OTTHUMP00000020345|hyaluronidase in meningioma|nuclear cytoplasmic O-GlcNAcase and acetyltransferase
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Interactome
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Disease
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Publication Reference
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Massively parallel identification of mRNA localization elements in primary cortical neurons.
Samantha Mendonsa, Nicolai von Kügelgen, Sayaka Dantsuji, Maya Ron, Laura Breimann, Artem Baranovskii, Inga Lödige, Marieluise Kirchner, Meret Fischer, Nadja Zerna, Lucija Bujanic, Philipp Mertins, Igor Ulitsky, Marina Chekulaeva.
Nature Neuroscience 2023 Mar; 26(3):394.
Application：WB-Ti, Mouse, Mouse coritcal neurons.
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Massively parallel identification of mRNA localization elements in primary cortical neurons.
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