PDLIM5 monoclonal antibody (M01), clone 3E11-F6
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More Files
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Western Blot (Cell lysate)
PDLIM5 monoclonal antibody (M01), clone 3E11-F6. Western Blot analysis of PDLIM5 expression in HepG2.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PDLIM5 is approximately 3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PDLIM5 on A-431 cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (91.3 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PDLIM5.
Immunogen
PDLIM5 (AAH08741, 1 a.a. ~ 596 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKAAQANVRIGDVVLSIDGINAQGMTHLEAQNKIKGCTGSLNMTLQRASAAPKPEPVPVQKGEPKEVVKPVPITSPAVSKVTSTNNMAYNKAPRPFGSVSSPKVTSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVTSVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKKRLIEDTEDWRPRTGTTQSRSFRILAQITGTEHLKESEADNTKKANNSQEPSPQLASSVASTRSMPESLDSPTSGRPGVTSLTTAAAFKPVGSTGVIKSPSWQRPNQGVPSTGRISNSAAYSGSVAPANSALGQTQPSDQDTLVQRAEHIPAGKRTPMCAHCNQVIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEEKGALYCELCYEKFFAPECGRCQRKILGEVINALKQTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYYALFGTICHGCEFPIEAGDMFLEALGYTWHDTCFVCSVCCESLEGQTFFSKKDKPLCKKHAHSVNF
Host
Mouse
Reactivity
Human
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (91.3 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PDLIM5 monoclonal antibody (M01), clone 3E11-F6. Western Blot analysis of PDLIM5 expression in HepG2.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PDLIM5 is approximately 3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PDLIM5 on A-431 cell. [antibody concentration 10 ug/ml] -
Gene Info — PDLIM5
Entrez GeneID
10611GeneBank Accession#
BC008741Protein Accession#
AAH08741Gene Name
PDLIM5
Gene Alias
ENH, ENH1, L9, LIM
Gene Description
PDZ and LIM domain 5
Omim ID
605904Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized. [provided by RefSeq
Other Designations
enigma homolog|enigma-like LIM domain protein
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Interactome
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Disease
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Publication Reference
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Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments.
Xiaolan Huang, Rongmei Qu, Yan Peng, Yuchao Yang, Tingyu Fan, Bing Sun, Asmat Ullah Khan, Shutong Wu, Kuanhai Wei, Chujiang Xu, Jingxing Dai, Jun Ouyang, Shizhen Zhong.
Biomolecules 2021 May; 11(5):759.
Application：IF, WB-Tr, Human, Human skin fibroblasts.
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Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments.
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