SC65 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SC65 transfected lysate.
Western Blot
Lane 1: SC65 transfected lysate ( 48.18 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SC65 full-length
Host
Human
Theoretical MW (kDa)
48.18
Interspecies Antigen Sequence
Mouse (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SC65 antibody (H00010609-B01) by Western Blots.
SDS-PAGE Gel
SC65 transfected lysate.
Western Blot
Lane 1: SC65 transfected lysate ( 48.18 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SC65
Entrez GeneID
10609GeneBank Accession#
NM_006455Protein Accession#
NP_006446Gene Name
SC65
Gene Alias
NOL55
Gene Description
synaptonemal complex protein SC65
Gene Ontology
HyperlinkGene Summary
This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq
Other Designations
OTTHUMP00000164758|nucleolar autoantigen No55|nucleolar autoantigen, 55kDa
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