AGPAT2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AGPAT2 transfected lysate.
Western Blot
Lane 1: AGPAT2 transfected lysate ( 30.69 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AGPAT2 full-length
Host
Human
Theoretical MW (kDa)
30.69
Interspecies Antigen Sequence
Mouse (76); Rat (75)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AGPAT2 antibody (H00010555-B01) by Western Blots.
SDS-PAGE Gel
AGPAT2 transfected lysate.
Western Blot
Lane 1: AGPAT2 transfected lysate ( 30.69 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AGPAT2
Entrez GeneID
10555GeneBank Accession#
NM_006412.3Protein Accession#
NP_006403.2Gene Name
AGPAT2
Gene Alias
1-AGPAT2, BSCL, BSCL1, LPAAB, LPAAT-beta
Gene Description
1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
1-AGP acyltransferase 2|1-acyl-sn-glycerol-3-phosphate acyltransferase beta|1-acylglycerol-3-phosphate O-acyltransferase 2|1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase-beta)|OTTHUMP00000022595|OTTHUMP00000022596|ly
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Interactome
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Pathway
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