SAP18 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SAP18 full-length
Host
Human
Theoretical MW (kDa)
16.94
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SAP18 antibody (H00010284-B01) by Western Blots.
SDS-PAGE Gel
SAP18 transfected lysate.
Western Blot
Lane 1: SAP18 transfected lysate ( 16.94 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SAP18
Entrez GeneID
10284GeneBank Accession#
NM_005870.3Protein Accession#
-Gene Name
SAP18
Gene Alias
2HOR0202, MGC27131, SAP18P
Gene Description
Sin3A-associated protein, 18kDa
Omim ID
602949Gene Ontology
HyperlinkGene Summary
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq
Other Designations
OTTHUMP00000018107|cell growth inhibiting protein 38|histone deacetlyase complex subunit SAP18|sin3-associated polypeptide, p18
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