MRPS31 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPS31 full-length
Host
Human
Theoretical MW (kDa)
45.3
Interspecies Antigen Sequence
Mouse (64); Rat (65)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPS31 antibody (H00010240-B01) by Western Blots.
SDS-PAGE Gel
MRPS31 transfected lysate.
Western Blot
Lane 1: MRPS31 transfected lysate ( 45.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPS31
Entrez GeneID
10240GeneBank Accession#
BC022045Protein Accession#
AAH22045Gene Name
MRPS31
Gene Alias
IMOGN38, MRP-S31, S31mt
Gene Description
mitochondrial ribosomal protein S31
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13. [provided by RefSeq
Other Designations
OTTHUMP00000018305|imogen 38
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