ACTR1B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ACTR1B transfected lysate.
Western Blot
Lane 1: ACTR1B transfected lysate ( 41.47 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACTR1B full-length
Host
Human
Theoretical MW (kDa)
41.47
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACTR1B antibody (H00010120-B01) by Western Blots.
SDS-PAGE Gel
ACTR1B transfected lysate.
Western Blot
Lane 1: ACTR1B transfected lysate ( 41.47 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACTR1B
Entrez GeneID
10120GeneBank Accession#
NM_005735.2Protein Accession#
-Gene Name
ACTR1B
Gene Alias
ARP1B, CTRN2, PC3
Gene Description
ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Omim ID
605144Gene Ontology
HyperlinkGene Summary
This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq
Other Designations
ARP1 actin-related protein 1 homolog B, centractin beta|centractin beta
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