ACOT8 monoclonal antibody (M03), clone 3F1
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More Files
- More Functions
Western Blot (Cell lysate)
ACOT8 monoclonal antibody (M03), clone 3F1. Western Blot analysis of ACOT8 expression in A-431(Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of ACOT8 expression in transfected 293T cell line by ACOT8 monoclonal antibody (M03), clone 3F1.
Lane 1: ACOT8 transfected lysate (Predicted MW: 35.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACOT8 is 1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.62 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ACOT8.
Immunogen
ACOT8 (NP_005460.2, 108 a.a. ~ 215 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SVRSVKAVQHGKPIFICQASFQQAQPSPMQHQFSMPTVPPPEELLDCETLIDQYLRDPNLQKRYPLALNRIAAQEVPIEIKPVNPSPLSQLQRMEPKQMFWVRARGYI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (82)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.62 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ACOT8 monoclonal antibody (M03), clone 3F1. Western Blot analysis of ACOT8 expression in A-431(Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ACOT8 expression in transfected 293T cell line by ACOT8 monoclonal antibody (M03), clone 3F1.
Lane 1: ACOT8 transfected lysate (Predicted MW: 35.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACOT8 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — ACOT8
Entrez GeneID
10005GeneBank Accession#
NM_005469Protein Accession#
NP_005460.2Gene Name
ACOT8
Gene Alias
HNAACTE, PTE-2, PTE1, PTE2, hACTE-III, hTE
Gene Description
acyl-CoA thioesterase 8
Omim ID
608123Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HIV-Nef associated acyl-CoA thioesterase|OTTHUMP00000031675|long-chain fatty-acyl-CoA hydrolase|palmitoyl-CoA hydrolase|peroxisomal acyl-CoA thioesterase 1|peroxisomal acyl-coenzyme A thioester hydrolase 1|peroxisomal long-chain acyl-CoA thioesterase 1|th
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