MTRF1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MTRF1 full-length
Host
Human
Theoretical MW (kDa)
18.2
Interspecies Antigen Sequence
Mouse (66); Rat (65)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MTRF1 antibody (H00009617-B01) by Western Blots.
SDS-PAGE Gel
MTRF1 transfected lysate.
Western Blot
Lane 1: MTRF1 transfected lysate ( 18.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MTRF1
Entrez GeneID
9617GeneBank Accession#
ENST00000239852Protein Accession#
ENSP00000239852Gene Name
MTRF1
Gene Alias
MGC47721, MRF1, MTTRF1, RF1
Gene Description
mitochondrial translational release factor 1
Omim ID
604601Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear. [provided by RefSeq
Other Designations
OTTHUMP00000018315|OTTHUMP00000018316|mitochontrial peptide chain release factor 1
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