KLF4 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human KLF4 full-length ORF ( NP_004226.2, 1 a.a. - 470 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
76.5
Interspecies Antigen Sequence
Mouse (89); Rat (90)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — KLF4
Entrez GeneID
9314GeneBank Accession#
NM_004235.3Protein Accession#
NP_004226.2Gene Name
KLF4
Gene Alias
EZF, GKLF
Gene Description
Kruppel-like factor 4 (gut)
Omim ID
602253Gene Ontology
HyperlinkOther Designations
OTTHUMP00000021862|endothelial Kruppel-like zinc finger protein
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Interactome
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Disease
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Publication Reference
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Delivery of reprogramming factors into fibroblasts for generation of non-genetic induced pluripotent stem cells using a cationic bolaamphiphile as a non-viral vector.
Khan M, Narayanan K, Lu H, Choo Y, Du C, Wiradharma N, Yang YY, Wan AC.
Biomaterials 2013 Jul; 34(21):5336.
Application:Func, Human, Foreskin fibroblasts.
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DLK2 is a transcriptional target of KLF4 in the early stages of adipogenesis.
Rivero S, Díaz-Guerra MJ, Monsalve EM, Laborda J, García-Ramírez JJ.
Journal of Molecular Biology 2012 Mar; 417(1-2):36.
Application:EMSA, Mouse, 3T3-L1 cells.
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Delivery of reprogramming factors into fibroblasts for generation of non-genetic induced pluripotent stem cells using a cationic bolaamphiphile as a non-viral vector.
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