PSCD1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PSCD1 full-length
Host
Human
Theoretical MW (kDa)
43.89
Interspecies Antigen Sequence
Mouse (96); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PSCD1 antibody (H00009267-B01) by Western Blots.
SDS-PAGE Gel
PSCD1 transfected lysate.
Western Blot
Lane 1: PSCD1 transfected lysate ( 43.89 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYTH1
Entrez GeneID
9267GeneBank Accession#
NM_004762.2Protein Accession#
NP_004753.1Gene Name
CYTH1
Gene Alias
B2-1, CYTOHESIN-1, D17S811E, FLJ34050, FLJ41900, PSCD1, SEC7
Gene Description
cytohesin 1
Omim ID
182115Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. The encoded protein is 83% homologous to that of CYTH2. [provided by RefSeq
Other Designations
OTTHUMP00000196778|cytoadhesin 1|homolog of secretory protein SEC7|pleckstrin homology, Sec7 and coiled-coil domains 1
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