PIGQ monoclonal antibody (M02), clone 2B7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PIGQ.
Immunogen
PIGQ (NP_683721.1, 661 a.a. ~ 758 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
HCPMPTLCTQVQRVRPPQQPQVEGWSPWGLPSGSALAVGVEGPCQDEPPSPRHPLAPSAEQHPASGGLKQSLTPVPSGPGPSLPEPHGVYLRMFPGEV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (83)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PIGQ expression in transfected 293T cell line by PIGQ monoclonal antibody (M02), clone 2B7.
Lane 1: PIGQ transfected lysate(84.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PIGQ on formalin-fixed paraffin-embedded human placenta. [antibody concentration 4 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PIGQ is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — PIGQ
Entrez GeneID
9091GeneBank Accession#
NM_148920Protein Accession#
NP_683721.1Gene Name
PIGQ
Gene Alias
GPI1, MGC12693, c407A10.1, hGPI1
Gene Description
phosphatidylinositol glycan anchor biosynthesis, class Q
Omim ID
605754Gene Ontology
HyperlinkGene Summary
This gene is involved in the first step in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a N-acetylglucosaminyl transferase component that is part of the complex that catalyzes transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). [provided by RefSeq
Other Designations
N-acetylglucosaminyl transferase component Gpi1|N-acetylglucosamyl transferase component GPI1|c407A10.1 (GPI1 (N-acetylglucosaminyl transferase component))|phosphatidylinositol glycan, class Q
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Interactome
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Pathway
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Disease
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