RIOK3 monoclonal antibody (M01), clone 4A12-1D2
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More Files
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Western Blot (Cell lysate)
RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Western Blot analysis of RIOK3 expression in MCF-7.
Western Blot (Transfected lysate)
Western Blot analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M01), clone 4A12-1D2.
Lane 1: RIOK3 transfected lysate(59.1 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RIOK3 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RIOK3 is approximately 0.3ng/ml as a capture antibody.
Western Blot detection against Immunogen (82.5 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant RIOK3.
Immunogen
RIOK3 (AAH39729, 1 a.a. ~ 516 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (82.5 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Western Blot analysis of RIOK3 expression in MCF-7.Western Blot (Transfected lysate)
Western Blot analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M01), clone 4A12-1D2.
Lane 1: RIOK3 transfected lysate(59.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RIOK3 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RIOK3 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — RIOK3
Entrez GeneID
8780GeneBank Accession#
BC039729Protein Accession#
AAH39729Gene Name
RIOK3
Gene Alias
DKFZp779L1370, SUDD
Gene Description
RIO kinase 3 (yeast)
Omim ID
603579Gene Ontology
HyperlinkGene Summary
This gene was identified by the similarity of its product to the Aspergillus nidulans SUDD protein, an extragenic suppressor of the heat-sensitive bimD6 mutation that fails to attach properly to the spindle microtubules at a restrictive temperature. The specific function of this gene has not yet been determined. [provided by RefSeq
Other Designations
homolog of the Aspergillus nidulans sudD gene product|sudD (suppressor of bimD6, Aspergillus nidulans) homolog|sudD suppressor of Aspergillus nidulans bimD6 homolog|sudD suppressor of bimD6 homolog
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Interactome
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Publication Reference
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The atypical protein kinase RIOK3 contributes to glioma cell proliferation/survival, migration/invasion and the AKT/mTOR signaling pathway.
Zhang T, Ji D, Wang P, Dong L, Jin L, Shi H, Liu X, Meng Q, Yu R, Gao S.
Cancer Letters 2018 Feb; 415:151.
Application：WB-Ce, WB-Ti, Human, Human glioma tissues, U251 cells.
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Hypoxic regulation of RIOK3 is a major mechanism for cancer cell invasion and metastasis.
Singleton DC, Rouhi P, Zois CE, Haider S, Li JL, Kessler BM, Cao Y, Harris AL.
Oncogene 2015 Sep; 34(36):4713.
Application：IF, WB-Tr, Human, MDA-MB-231 cells.
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Genomic alterations link Rho family of GTPases to the highly invasive phenotype of pancreas cancer.
Kimmelman AC, Hezel AF, Aguirre AJ, Zheng H, Paik JH, Ying H, Chu GC, Zhang JX, Sahin E, Yeo G, Ponugoti A, Nabioullin R, Deroo S, Yang S, Wang X, McGrath JP, Protopopova M, Ivanova E, Zhang J, Feng B, Tsao MS, Redston M, Protopopov A, Xiao Y, Futreal PA,.
PNAS 2008 Dec; 105(49):19372.
Application：WB-Ce, Human, Human pancreatic ductal cells (HPDE).
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The atypical protein kinase RIOK3 contributes to glioma cell proliferation/survival, migration/invasion and the AKT/mTOR signaling pathway.
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