PRKRA monoclonal antibody (M01), clone 1B9-1A7
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More Files
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Western Blot (Tissue lysate)
PRKRA monoclonal antibody (M01), clone 1B9-1A7. Western Blot analysis of PRKRA expression in human liver.
Western Blot (Cell lysate)
PRKRA monoclonal antibody (M01), clone 1B9-1A7 Western Blot analysis of PRKRA expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody (M01), clone 1B9-1A7.
Lane 1: PRKRA transfected lysate(34.4 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. [antibody concentration 5 ug/ml]
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5 ug/ml]
Immunoprecipitation
Immunoprecipitation of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRKRA MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PRKRA is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (60.17 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PRKRA.
Immunogen
PRKRA (AAH09470, 1 a.a. ~ 313 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (60.17 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
PRKRA monoclonal antibody (M01), clone 1B9-1A7. Western Blot analysis of PRKRA expression in human liver.Western Blot (Cell lysate)
PRKRA monoclonal antibody (M01), clone 1B9-1A7 Western Blot analysis of PRKRA expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody (M01), clone 1B9-1A7.
Lane 1: PRKRA transfected lysate(34.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. [antibody concentration 5 ug/ml]Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5 ug/ml]Immunoprecipitation
Immunoprecipitation of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRKRA MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PRKRA is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — PRKRA
Entrez GeneID
8575GeneBank Accession#
BC009470Protein Accession#
AAH09470Gene Name
PRKRA
Gene Alias
DYT16, HSD14, PACT, RAX
Gene Description
protein kinase, interferon-inducible double stranded RNA dependent activator
Omim ID
603424Gene Ontology
HyperlinkGene Summary
This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
protein activator of the interferon-induced protein kinase
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Interactome
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Disease
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Publication Reference
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Knock-down of core proteins regulating microRNA biogenesis has no effect on sensitivity of lung cancer cells to ionizing radiation.
Surova O, Akbar NS, Zhivotovsky B.
PLoS One 2012 Mar; 7(3):e33134.
Application：WB-Ce, Human, Human lung cancer cell lines.
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Knock-down of core proteins regulating microRNA biogenesis has no effect on sensitivity of lung cancer cells to ionizing radiation.
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