NCK2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NCK2 transfected lysate
Western Blot
Lane 1: NCK2 transfected lysate ( 42.9 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NCK2 full-length
Host
Human
Theoretical MW (kDa)
42.9
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NCK2 antibody (H00008440-B01) by Western Blots.
SDS-PAGE Gel
NCK2 transfected lysate
Western Blot
Lane 1: NCK2 transfected lysate ( 42.9 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NCK2
Entrez GeneID
8440GeneBank Accession#
NM_001004720Protein Accession#
NP_001004720Gene Name
NCK2
Gene Alias
GRB4, NCKbeta
Gene Description
NCK adaptor protein 2
Omim ID
604930Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
SH2/SH3 adaptor protein NCK-beta|noncatalytic region of tyrosine kinase, beta
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Interactome
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Pathway
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Disease
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