MAPKAPK3 monoclonal antibody (M01), clone 3F4
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More Files
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Western Blot (Cell lysate)
MAPKAPK3 monoclonal antibody (M01), clone 3F4. Western Blot analysis of MAPKAPK3 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot (Cell lysate)
MAPKAPK3 monoclonal antibody (M01), clone 3F4 Western Blot analysis of MAPKAPK3 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of MAPKAPK3 expression in transfected 293T cell line by MAPKAPK3 monoclonal antibody (M01), clone 3F4.
Lane 1: MAPKAPK3 transfected lysate (Predicted MW: 43 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of MAPKAPK3 transfected lysate using anti-MAPKAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK3 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPKAPK3 is approximately 0.1ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MAPKAPK3.
Immunogen
MAPKAPK3 (AAH01662, 272 a.a. ~ 382 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (96); Rat (94)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MAPKAPK3 monoclonal antibody (M01), clone 3F4. Western Blot analysis of MAPKAPK3 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Cell lysate)
MAPKAPK3 monoclonal antibody (M01), clone 3F4 Western Blot analysis of MAPKAPK3 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of MAPKAPK3 expression in transfected 293T cell line by MAPKAPK3 monoclonal antibody (M01), clone 3F4.
Lane 1: MAPKAPK3 transfected lysate (Predicted MW: 43 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of MAPKAPK3 transfected lysate using anti-MAPKAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK3 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPKAPK3 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — MAPKAPK3
Entrez GeneID
7867GeneBank Accession#
BC001662Protein Accession#
AAH01662Gene Name
MAPKAPK3
Gene Alias
3PK, MAPKAP3
Gene Description
mitogen-activated protein kinase-activated protein kinase 3
Omim ID
602130Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. [provided by RefSeq
Other Designations
MAPKAP kinase 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Cooperative and distinct functions of MK2 and MK3 in the regulation of the macrophage transcriptional response to lipopolysaccharide.
Ehlting C, Rex J, Albrecht U, Deenen R, Tiedje C, Köhrer K, Sawodny O, Gaestel M, Häussinger D, Bode JG.
Scientific Reports 2019 Jul; 9(1):11021.
Application：WB, Mouse, Bone marrow-derived macrophages.
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Label-Free Nanoplasmonic Biosensing of Cancer Biomarkers for Clinical Diagnosis.
Portela A, Peláez EC, Calvo-Lozano O, Estévez MC, Lechuga LM.
Methods in Molecular Biology (Clifton, N.J.) 2019 Jul; 2027:115.
Application：Func, Human, Human plasma.
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Cooperative and distinct functions of MK2 and MK3 in the regulation of the macrophage transcriptional response to lipopolysaccharide.
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