ZP2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ZP2 transfected lysate.
Western Blot
Lane 1: ZP2 transfected lysate ( 81.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ZP2 full-length
Host
Human
Theoretical MW (kDa)
81.3
Interspecies Antigen Sequence
Mouse (57); Rat (57)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ZP2 antibody (H00007783-B01) by Western Blots.
SDS-PAGE Gel
ZP2 transfected lysate.
Western Blot
Lane 1: ZP2 transfected lysate ( 81.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ZP2
Entrez GeneID
7783GeneBank Accession#
BC096305Protein Accession#
AAH96305Gene Name
ZP2
Gene Alias
ZPA
Gene Description
zona pellucida glycoprotein 2 (sperm receptor)
Omim ID
182888Gene Ontology
HyperlinkGene Summary
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. [provided by RefSeq
Other Designations
zona pellucida glycoprotein 2|zona pellucida protein A|zona pellucida sperm-binding protein 2
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