WNT3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
WNT3 transfected lysate.
Western Blot
Lane 1: WNT3 transfected lysate ( 39.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WNT3 full-length
Host
Human
Theoretical MW (kDa)
39.6
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WNT3 antibody (H00007473-D01P) by Western Blots.
SDS-PAGE Gel
WNT3 transfected lysate.
Western Blot
Lane 1: WNT3 transfected lysate ( 39.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WNT3
Entrez GeneID
7473GeneBank Accession#
NM_030753Protein Accession#
NP_110380.1Gene Name
WNT3
Gene Alias
INT4, MGC131950, MGC138321, MGC138323
Gene Description
wingless-type MMTV integration site family, member 3
Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq
Other Designations
WNT-3 proto-oncogene protein
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Interactome
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Pathway
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Disease
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