CCT3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CCT3 transfected lysate.
Western Blot
Lane 1: CCT3 transfected lysate ( 60.40 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CCT3 full-length
Host
Human
Theoretical MW (kDa)
60.4
Interspecies Antigen Sequence
Mouse (97); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CCT3 antibody (H00007203-B01) by Western Blots.
SDS-PAGE Gel
CCT3 transfected lysate.
Western Blot
Lane 1: CCT3 transfected lysate ( 60.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CCT3
Entrez GeneID
7203GeneBank Accession#
BC008019.1Protein Accession#
AAH08019.1Gene Name
CCT3
Gene Alias
CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5
Gene Description
chaperonin containing TCP1, subunit 3 (gamma)
Omim ID
600114Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000025735|T-complex protein 1, gamma subunit|TCP1 (t-complex-1) ring complex, polypeptide 5|chaperonin containing TCP1, subunit 3
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