TRAF1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRAF1 full-length
Host
Human
Theoretical MW (kDa)
45.87
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRAF1 antibody (H00007185-B01) by Western Blots.
SDS-PAGE Gel
TRAF1 transfected lysate.
Western Blot
Lane 1: TRAF1 transfected lysate ( 45.87 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRAF1
Entrez GeneID
7185GeneBank Accession#
NM_005658Protein Accession#
NP_005649Gene Name
TRAF1
Gene Alias
EBI6, MGC:10353
Gene Description
TNF receptor-associated factor 1
Omim ID
601711Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. [provided by RefSeq
Other Designations
Epstein-Bar virus-induced protein 6|OTTHUMP00000022000
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