SURF5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MED22 transfected lysate.
Western Blot
Lane 1: MED22 transfected lysate ( 16.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SURF5 full-length
Host
Human
Theoretical MW (kDa)
16.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SURF5 antibody (H00006837-B01) by Western Blots.
SDS-PAGE Gel
MED22 transfected lysate.
Western Blot
Lane 1: MED22 transfected lysate ( 16.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MED22
Entrez GeneID
6837GeneBank Accession#
NM_181491Protein Accession#
NP_852468Gene Name
MED22
Gene Alias
MED24, MGC48682, SURF5
Gene Description
mediator complex subunit 22
Omim ID
185641Gene Ontology
HyperlinkGene Summary
This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000022480|surfeit 5|surfeit locus protein 5
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Interactome
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Disease
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