SRY 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SRY transfected lysate.
Western Blot
Lane 1: SRY transfected lysate ( 22.55 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SRY full-length
Host
Human
Theoretical MW (kDa)
22.55
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SRY antibody (H00006736-B01) by Western Blots.
SDS-PAGE Gel
SRY transfected lysate.
Western Blot
Lane 1: SRY transfected lysate ( 22.55 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SRY
Entrez GeneID
6736GeneBank Accession#
NM_003140.1Protein Accession#
-Gene Name
SRY
Gene Alias
TDF, TDY
Gene Description
sex determining region Y
Omim ID
480000Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000034324|essential protein for sex determination in human males|sex determining region protein|sex-determining region on Y|testis-determining factor
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Interactome
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Disease
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