SP2 monoclonal antibody (M01), clone 5D3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of SP2 expression in transfected 293T cell line by SP2 monoclonal antibody (M01), clone 5D3.
Lane 1: SP2 transfected lysate (Predicted MW: 25.6 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SP2 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (35.75 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SP2.
Immunogen
SP2 (NP_003101.2, 71 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SPGKNSFGILSSKGNILQIQGSQLSASYPGGQLVFAIQNPTMINKGTRSNANIQYQAVPQIQASNSQTIQVQPNLTNQIQIIPGTNQAIIT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (36)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SP2 expression in transfected 293T cell line by SP2 monoclonal antibody (M01), clone 5D3.
Lane 1: SP2 transfected lysate (Predicted MW: 25.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SP2 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — SP2
Entrez GeneID
6668GeneBank Accession#
NM_003110Protein Accession#
NP_003101.2Gene Name
SP2
Gene Alias
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Gene Description
Sp2 transcription factor
Omim ID
601801Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. [provided by RefSeq
Other Designations
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Interactome
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