RPS27A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RPS27A full-length
Host
Human
Theoretical MW (kDa)
18
Interspecies Antigen Sequence
Mouse (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RPS27A antibody (H00006233-D01P) by Western Blots.
SDS-PAGE Gel
RPS27A transfected lysate.
Western Blot
Lane 1: RPS27A transfected lysate ( 18.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RPS27A
Entrez GeneID
6233GeneBank Accession#
NM_002954.3Protein Accession#
NP_002945.1Gene Name
RPS27A
Gene Alias
CEP80, HUBCEP80, UBA80, UBCEP1, UBCEP80
Gene Description
ribosomal protein S27a
Omim ID
191343Gene Ontology
HyperlinkGene Summary
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq
Other Designations
40S ribosomal protein S27a|ubiquitin and ribosomal protein S27a|ubiquitin carboxyl extension protein 80|ubiquitin-CEP80
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