MRPL12 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL12 full-length
Host
Human
Theoretical MW (kDa)
21.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL12 antibody (H00006182-B01) by Western Blots.
SDS-PAGE Gel
MRPL12 transfected lysate.
Western Blot
Lane 1: MRPL12 transfected lysate ( 21.89 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL12
Entrez GeneID
6182GeneBank Accession#
NM_002949Protein Accession#
NP_002940.2Gene Name
MRPL12
Gene Alias
5c5-2, FLJ60124, L12mt, MGC8610, MRP-L31/34, MRPL7, MRPL7/L12, RPML12
Gene Description
mitochondrial ribosomal protein L12
Omim ID
602375Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq
Other Designations
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