PTK9 monoclonal antibody (M02), clone 1E2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PTK9.
Immunogen
PTK9 (AAH22344, 80 a.a. ~ 252 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSSAKPKGPAGKRGIRRLIRGPAETEATTD
Host
Mouse
Reactivity
Human, Rat
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (44.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PTK9 monoclonal antibody (M02), clone 1E2 Western Blot analysis of PTK9 expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
PTK9 monoclonal antibody (M02), clone 1E2. Western Blot analysis of PTK9 expression in PC-12 ( Cat # L012V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PTK9 on formalin-fixed paraffin-embedded human ovary. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of TWF1 transfected lysate using anti-TWF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TWF1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PTK9 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — TWF1
Entrez GeneID
5756GeneBank Accession#
BC022344Protein Accession#
AAH22344Gene Name
TWF1
Gene Alias
A6, MGC23788, MGC41876, PTK9
Gene Description
twinfilin, actin-binding protein, homolog 1 (Drosophila)
Omim ID
610932Gene Ontology
HyperlinkGene Summary
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq
Other Designations
A6 protein tyrosine kinase|PTK9 protein tyrosine kinase 9|protein tyrosine kinase 9|twinfilin 1
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Interactome
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Disease
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Publication Reference
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Genomic Profiling of MicroRNA and Messenger RNA Reveals Deregulated MicroRNA Expression in Prostate Cancer.
Ambs S, Prueitt RL, Yi M, Hudson RS, Howe TM, Petrocca F, Wallace TA, Liu CG, Volinia S, Calin GA, Yfantis HG, Stephens RM, Croce CM.
Cancer Research 2008 Aug; 68(15):6162.
Application:WB-Tr, Human, LNCaP, PC-3 cells.
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Genomic Profiling of MicroRNA and Messenger RNA Reveals Deregulated MicroRNA Expression in Prostate Cancer.
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