PSMB7 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PSMB7 full-length
Host
Human
Theoretical MW (kDa)
30.58
Interspecies Antigen Sequence
Mouse (96); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PSMB7 antibody (H00005695-B02) by Western Blots.
SDS-PAGE Gel
PSMB7 transfected lysate.
Western Blot
Lane 1: PSMB7 transfected lysate ( 30.58 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PSMB7
Entrez GeneID
5695GeneBank Accession#
BC000509Protein Accession#
AAH00509Gene Name
PSMB7
Gene Alias
Z
Gene Description
proteasome (prosome, macropain) subunit, beta type, 7
Omim ID
604030Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit). [provided by RefSeq
Other Designations
OTTHUMP00000022798|macropain chain Z|multicatalytic endopeptidase complex chain Z|proteasome beta 7 subunit|proteasome catalytic subunit 2|proteasome subunit Z|proteasome subunit alpha|proteasome subunit beta 7
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