POLR2E 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00005434-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

POLR2E transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: POLR2E transfected lysate ( 23.21 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-POLR2E full-length

    Host

    Human

    Theoretical MW (kDa)

    23.21

    Interspecies Antigen Sequence

    Mouse (99); Rat (99)

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-POLR2E antibody (H00005434-B01) by Western Blots.

    SDS-PAGE Gel

    POLR2E transfected lysate.

    Western Blot

    Lane 1: POLR2E transfected lysate ( 23.21 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — POLR2E

    Entrez GeneID

    5434

    GeneBank Accession#

    NM_002695.2

    Protein Accession#

    NP_002686.2

    Gene Name

    POLR2E

    Gene Alias

    RPABC1, RPB5, XAP4, hRPB25, hsRPB5

    Gene Description

    polymerase (RNA) II (DNA directed) polypeptide E, 25kDa

    Omim ID

    180664

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq

    Other Designations

    DNA directed RNA polymerase II 23 kda polypeptide|DNA directed RNA polymerase II polypeptide E|polymerase (RNA) II (DNA directed) polypeptide E (25kD)

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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