PHF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PHF1 transfected lysate.
Western Blot
Lane 1: PHF1 transfected lysate ( 62.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PHF1 full-length
Host
Human
Theoretical MW (kDa)
62.1
Interspecies Antigen Sequence
Mouse (92); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PHF1 antibody (H00005252-D01P) by Western Blots.
SDS-PAGE Gel
PHF1 transfected lysate.
Western Blot
Lane 1: PHF1 transfected lysate ( 62.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PHF1
Entrez GeneID
5252GeneBank Accession#
NM_024165.1Protein Accession#
NP_077084.1Gene Name
PHF1
Gene Alias
MTF2L2, PCL1, PHF2
Gene Description
PHD finger protein 1
Omim ID
602881Gene Ontology
HyperlinkGene Summary
This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000029139|OTTHUMP00000029140
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Interactome
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