PF4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PF4 transfected lysate.
Western Blot
Lane 1: PF4 transfected lysate ( 10.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PF4 full-length
Host
Human
Theoretical MW (kDa)
10.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PF4 antibody (H00005196-D01P) by Western Blots.
SDS-PAGE Gel
PF4 transfected lysate.
Western Blot
Lane 1: PF4 transfected lysate ( 10.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PF4
Entrez GeneID
5196GeneBank Accession#
NM_002619Protein Accession#
NP_002610.1Gene Name
PF4
Gene Alias
CXCL4, MGC138298, SCYB4
Gene Description
platelet factor 4
Omim ID
173460Gene Ontology
HyperlinkGene Summary
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM
Other Designations
chemokine (C-X-C motif) ligand 4|platelet factor 4 (chemokine (C-X-C motif) ligand 4)
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