PDPK1 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Cell lysate)
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in NIH/3T3.
Western Blot (Cell lysate)
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in K-562.
Western Blot (Cell lysate)
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in Raw 264.7.
Western Blot (Transfected lysate)
Western Blot analysis of PDPK1 expression in transfected 293T cell line (H00005170-T01) by PDPK1 MaxPab polyclonal antibody.
Lane 1: PDPK1 transfected lysate(47.19 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between RPS6KA3 and PDPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human PDPK1 protein.
Immunogen
PDPK1 (NP_112558.2, 1 a.a. ~ 429 a.a) full-length human protein.
Sequence
MARTTSQLYDAVPIQSSVVLCSCPSPSMVRTQTESSTPPGIPGGSRQGPAMDGTAAEPRPGAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYATRANSFVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTAYLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ
Host
Mouse
Reactivity
Human, Mouse
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in NIH/3T3.Western Blot (Cell lysate)
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in K-562.Western Blot (Cell lysate)
PDPK1 MaxPab polyclonal antibody. Western Blot analysis of PDPK1 expression in Raw 264.7.Western Blot (Transfected lysate)
Western Blot analysis of PDPK1 expression in transfected 293T cell line (H00005170-T01) by PDPK1 MaxPab polyclonal antibody.
Lane 1: PDPK1 transfected lysate(47.19 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between RPS6KA3 and PDPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — PDPK1
Entrez GeneID
5170GeneBank Accession#
NM_031268Protein Accession#
NP_112558.2Gene Name
PDPK1
Gene Alias
MGC20087, MGC35290, PDK1, PRO0461
Gene Description
3-phosphoinositide dependent protein kinase-1
Omim ID
605213Gene Ontology
HyperlinkOther Designations
PkB kinase like gene 1|PkB-like 1|protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY.
Molecular & Cellular Proteomics 2013 May; 12(5):1335.
Application：Profiling, Human, Huh7 cells, Mahlavu cells.
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An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
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