ENPP1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ENPP1 full-length
Host
Human
Theoretical MW (kDa)
96.14
Interspecies Antigen Sequence
Mouse (81); Rat (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ENPP1 antibody (H00005167-B01) by Western Blots.
Western Blot
Lane 1: ENPP1 transfected lysate ( 99.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
ENPP1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ENPP1
Entrez GeneID
5167GeneBank Accession#
NM_006208Protein Accession#
NP_006199Gene Name
ENPP1
Gene Alias
M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1
Gene Description
ectonucleotide pyrophosphatase/phosphodiesterase 1
Gene Ontology
HyperlinkGene Summary
This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq
Other Designations
Ly-41 antigen|OTTHUMP00000043194|alkaline phosphodiesterase 1|membrane component, chromosome 6, surface marker 1|phosphodiesterase I/nucleotide pyrophosphatase 1|plasma-cell membrane glycoprotein 1
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Interactome
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Disease
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