PCCA 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00005095-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

PCCA transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: PCCA transfected lysate ( 77.44 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-PCCA full-length

    Host

    Human

    Theoretical MW (kDa)

    77.44

    Interspecies Antigen Sequence

    Mouse (91); Rat (90)

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-PCCA antibody (H00005095-B01) by Western Blots.

    SDS-PAGE Gel

    PCCA transfected lysate.

    Western Blot

    Lane 1: PCCA transfected lysate ( 77.44 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — PCCA

    Entrez GeneID

    5095

    GeneBank Accession#

    BC000140.2

    Protein Accession#

    AAH00140.1

    Gene Name

    PCCA

    Gene Alias

    -

    Gene Description

    propionyl Coenzyme A carboxylase, alpha polypeptide

    Omim ID

    232000 606054

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

    Other Designations

    OTTHUMP00000018635|PCCase alpha subunit|pccA complementation group|propanoyl-CoA:carbon dioxide ligase alpha subunit|propionyl-CoA carboxylase alpha chain, mitochondrial|propionyl-Coenzyme A carboxylase, alpha polypeptide

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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