P4HA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
P4HA1 transfected lysate.
Western Blot
Lane 1: P4HA1 transfected lysate ( 58.85 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-P4HA1 full-length
Host
Human
Theoretical MW (kDa)
58.85
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-P4HA1 antibody (H00005033-B01) by Western Blots.
SDS-PAGE Gel
P4HA1 transfected lysate.
Western Blot
Lane 1: P4HA1 transfected lysate ( 58.85 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — P4HA1
Entrez GeneID
5033GeneBank Accession#
NM_001017962.1Protein Accession#
NP_001017962.1Gene Name
P4HA1
Gene Alias
P4HA
Gene Description
prolyl 4-hydroxylase, alpha polypeptide I
Omim ID
176710Gene Ontology
HyperlinkGene Summary
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
C-P4Halpha(I)|collagen prolyl 4-hydroxylase alpha(I)|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I|prolyl 4-hydroxylase, alpha I subunit
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Interactome
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Pathway
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Disease
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