NOS3 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NOS3 full-length
Host
Human
Theoretical MW (kDa)
133.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NOS3 antibody (H00004846-B01) by Western Blots.
SDS-PAGE Gel
NOS3 transfected lysate.
Western Blot
Lane 1: NOS3 transfected lysate ( 133.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NOS3
Entrez GeneID
4846GeneBank Accession#
BC069465.1Protein Accession#
-Gene Name
NOS3
Gene Alias
ECNOS, eNOS
Gene Description
nitric oxide synthase 3 (endothelial cell)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
endothelial nitric oxidase synthase|endothelial nitric oxide synthase
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