NODAL monoclonal antibody (M03J), clone 5C3
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NODAL.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
NODAL (NP_060525, 275 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGC
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Preparation Method
Cell Culture Production
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NODAL monoclonal antibody (M03J), clone 5C3. Western Blot analysis of NODAL expression in PC-12.Western Blot (Cell lysate)
NODAL monoclonal antibody (M03J), clone 5C3. Western Blot analysis of NODAL expression in Jurkat.Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NODAL is 3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NODAL on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — NODAL
Entrez GeneID
4838GeneBank Accession#
NM_018055Protein Accession#
NP_060525Gene Name
NODAL
Gene Alias
MGC138230
Gene Description
nodal homolog (mouse)
Omim ID
601265Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq
Other Designations
OTTHUMP00000019754|nodal|nodal, mouse, homolog
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