NME1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NME1 transfected lysate.
Western Blot
Lane 1: NME1 transfected lysate ( 19.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NME1 full-length
Host
Human
Theoretical MW (kDa)
19.7
Interspecies Antigen Sequence
Mouse (94); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NME1 antibody (H00004830-D01P) by Western Blots.
SDS-PAGE Gel
NME1 transfected lysate.
Western Blot
Lane 1: NME1 transfected lysate ( 19.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NME1
Entrez GeneID
4830GeneBank Accession#
NM_198175Protein Accession#
ENSP00000337060Gene Name
NME1
Gene Alias
AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1
Gene Description
non-metastatic cells 1, protein (NM23A) expressed in
Gene Ontology
HyperlinkGene Summary
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
Other Designations
NDP kinase A|OTTHUMP00000174772
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Interactome
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Pathway
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