NFIL3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NFIL3 transfected lysate.
Western Blot
Lane 1: NFIL3 transfected lysate ( 50.93 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NFIL3 full-length
Host
Human
Theoretical MW (kDa)
50.93
Interspecies Antigen Sequence
Mouse (85); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NFIL3 antibody (H00004783-B01) by Western Blots.
SDS-PAGE Gel
NFIL3 transfected lysate.
Western Blot
Lane 1: NFIL3 transfected lysate ( 50.93 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NFIL3
Entrez GeneID
4783GeneBank Accession#
NM_005384.2Protein Accession#
NP_005375.2Gene Name
NFIL3
Gene Alias
E4BP4, IL3BP1, NF-IL3A, NFIL3A
Gene Description
nuclear factor, interleukin 3 regulated
Omim ID
605327Gene Ontology
HyperlinkGene Summary
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM
Other Designations
OTTHUMP00000021633|interleukin-3 promoter transcriptional activator
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Interactome
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