NEFL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
NEFL transfected lysate.
Western Blot
Lane 1: NEFL transfected lysate ( 59.84 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NEFL full-length
Host
Human
Theoretical MW (kDa)
59.84
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NEFL antibody (H00004747-B01) by Western Blots.
SDS-PAGE Gel
NEFL transfected lysate.
Western Blot
Lane 1: NEFL transfected lysate ( 59.84 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NEFL
Entrez GeneID
4747GeneBank Accession#
NM_006158.2Protein Accession#
-Gene Name
NEFL
Gene Alias
CMT1F, CMT2E, FLJ53642, NF-L, NF68, NFL
Gene Description
neurofilament, light polypeptide
Gene Ontology
HyperlinkGene Summary
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq
Other Designations
light molecular weight neurofilament protein|neurofilament protein, light chain|neurofilament subunit NF-L|neurofilament triplet L protein|neurofilament, light polypeptide 68kDa|neurofilament-light
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Interactome
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Pathway
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Disease
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