NCF4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: NCF4 transfected lysate ( 39 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
NCF4 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NCF4 full-length
Host
Human
Theoretical MW (kDa)
37.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NCF4 antibody (H00004689-B01) by Western Blots.
Western Blot
Lane 1: NCF4 transfected lysate ( 39 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
NCF4 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NCF4
Entrez GeneID
4689GeneBank Accession#
NM_000631Protein Accession#
NP_000622Gene Name
NCF4
Gene Alias
MGC3810, NCF, P40PHOX, SH3PXD4
Gene Description
neutrophil cytosolic factor 4, 40kDa
Omim ID
601488Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000028736|OTTHUMP00000043756|neutrophil NADPH oxidase factor 4|neutrophil cytosol factor 4|neutrophil cytosolic factor 4
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