NCBP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NCBP1 transfected lysate.
Western Blot
Lane 1: NCBP1 transfected lysate ( 87.01 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NCBP1 full-length
Host
Human
Theoretical MW (kDa)
87.01
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NCBP1 antibody (H00004686-B01) by Western Blots.
SDS-PAGE Gel
NCBP1 transfected lysate.
Western Blot
Lane 1: NCBP1 transfected lysate ( 87.01 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NCBP1
Entrez GeneID
4686GeneBank Accession#
NM_002486.4Protein Accession#
NP_002477.1Gene Name
NCBP1
Gene Alias
CBP80, MGC2087, NCBP
Gene Description
nuclear cap binding protein subunit 1, 80kDa
Omim ID
600469Gene Ontology
HyperlinkGene Summary
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq
Other Designations
OTTHUMP00000021753
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