MECP2 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human MECP2 full-length ORF ( NP_004983.1, 1 a.a. - 486 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
78.8
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — MECP2
Entrez GeneID
4204GeneBank Accession#
NM_004992.2Protein Accession#
NP_004983.1Gene Name
MECP2
Gene Alias
AUTSX3, DKFZp686A24160, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT
Gene Description
methyl CpG binding protein 2 (Rett syndrome)
Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq
Other Designations
OTTHUMP00000026021|methyl CpG binding protein 2
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Interactome
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Disease
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Publication Reference
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The Cell Adhesion Molecule L1 Interacts with Methyl CpG Binding Protein 2 via Its Intracellular Domain.
Gabriele Loers, Ralf Kleene, Maria Girbes Minguez, Melitta Schachner.
International Journal of Molecular Sciences 2022 Mar; 23(7):3554.
Application:ELISA, Human, Recombinant protein.
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Reading ADP-ribosylation signaling using chemical biology and interaction proteomics.
Katarzyna W Kliza, Qiang Liu, Laura W M Roosenboom, Pascal W T C Jansen, Dmitri V Filippov, Michiel Vermeulen.
Molecular Cell 2021 Nov; 81(21):4552.
Application:Purify, Human, Human Peptide.
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MeCP2 Regulates Gene Expression Through Recognition of H3K27me3.
Wooje Lee, Jeeho Kim, Jung-Mi Yun, Takbum Ohn, Qizhi Gong.
Nature Communications 2020 Jun; 11(1):3140.
Application:PI, Pull-Down, WB-Re, Recombinant proteins.
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An electrochemiluminescence based assay for quantitative detection of endogenous and exogenously applied MeCP2 protein variants.
Steinkellner H, Schönegger A, Etzler J, Kempaiah P, Huber A, Hahn K, Rose K, Duerr M, Christodoulou J, Beribisky AV, Neuhaus W, Laccone F.
Scientific Reports 2019 May; 9(1):7929.
Application:Func, Recombinant protein.
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Histone deacetylase 3 associates with MeCP2 to regulate FOXO and social behavior.
Nott A, Cheng J, Gao F, Lin YT, Gjoneska E, Ko T, Minhas P, Zamudio AV, Meng J, Zhang F, Jin P, Tsai LH.
Nature Neuroscience 2016 Nov; 19(11):1497.
Application:IP-WB, Recombinant protein.
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The Cell Adhesion Molecule L1 Interacts with Methyl CpG Binding Protein 2 via Its Intracellular Domain.
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