MCM6 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MCM6 transfected lysate.
Western Blot
Lane 1: MCM6 transfected lysate ( 90.42 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MCM6 full-length
Host
Human
Theoretical MW (kDa)
90.42
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MCM6 antibody (H00004175-B01) by Western Blots.
SDS-PAGE Gel
MCM6 transfected lysate.
Western Blot
Lane 1: MCM6 transfected lysate ( 90.42 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MCM6
Entrez GeneID
4175GeneBank Accession#
NM_005915Protein Accession#
NP_005906Gene Name
MCM6
Gene Alias
MCG40308, Mis5, P105MCM
Gene Description
minichromosome maintenance complex component 6
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. [provided by RefSeq
Other Designations
DNA replication licensing factor MCM6|MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe)|MIS5 homolog|minichromosome maintenance deficient (mis5, S. pombe) 6|minichromosome maintenance deficient 6 homolog
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Interactome
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Pathway
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Disease
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