LIMK2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
LIMK2 transfected lysate.
Western Blot
Lane 1: LIMK2 transfected lysate ( 75.57 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LIMK2 full-length
Host
Human
Theoretical MW (kDa)
75.57
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LIMK2 antibody (H00003985-B01) by Western Blots.
SDS-PAGE Gel
LIMK2 transfected lysate.
Western Blot
Lane 1: LIMK2 transfected lysate ( 75.57 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LIMK2
Entrez GeneID
3985GeneBank Accession#
NM_001031801.1Protein Accession#
NP_001026971.1Gene Name
LIMK2
Gene Alias
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Gene Description
LIM domain kinase 2
Omim ID
601988Gene Ontology
HyperlinkGene Summary
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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