ID4 polyclonal antibody (A01)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ID4 expression in transfected 293T cell line by ID4 polyclonal antibody (A01).
Lane1:ID4 transfected lysate(16.622 KDa).
Lane2:Non-transfected lysate.
Western Blot detection against Immunogen (31.72 KDa) .
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Specification
Product Description
Mouse polyclonal antibody raised against a partial recombinant ID4.
Immunogen
ID4 (NP_001537, 60 a.a. ~ 110 a.a) partial recombinant protein with GST tag.
Sequence
PALCLQCDMNDCYSRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQLALET
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100)
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (31.72 KDa) .
Storage Buffer
50 % glycerol
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ID4 expression in transfected 293T cell line by ID4 polyclonal antibody (A01).
Lane1:ID4 transfected lysate(16.622 KDa).
Lane2:Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — ID4
Entrez GeneID
3400GeneBank Accession#
NM_001546Protein Accession#
NP_001537Gene Name
ID4
Gene Alias
IDB4, bHLHb27
Gene Description
inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
Omim ID
600581Gene Ontology
HyperlinkGene Summary
Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM
Other Designations
OTTHUMP00000016081
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Interactome
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Pathway
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