HMGB3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
HMGB3 transfected lysate.
Western Blot
Lane 1: HMGB3 transfected lysate ( 22.11 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HMGB3 full-length
Host
Human
Theoretical MW (kDa)
22.11
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HMGB3 antibody (H00003149-B01) by Western Blots.
SDS-PAGE Gel
HMGB3 transfected lysate.
Western Blot
Lane 1: HMGB3 transfected lysate ( 22.11 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HMGB3
Entrez GeneID
3149GeneBank Accession#
NM_005342.2Protein Accession#
NP_005333.2Gene Name
HMGB3
Gene Alias
HMG2A, HMG4, MGC90319
Gene Description
high-mobility group box 3
Omim ID
300193Gene Ontology
HyperlinkGene Summary
HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM
Other Designations
OTTHUMP00000025865|high-mobility group (nonhistone chromosomal) protein 4|non-histone chromosomal protein
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